Plasmid

Part:BBa_K4941034:Design

Designed by: Xinyu Chen   Group: iGEM23_ZJFH-Nanjing   (2023-10-08)


pet28a- T7 promoter- KsXR- His tag- T7 terminator- f1 origin- ColE1 pBR322 origin- kan-lac I


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 686
    Illegal XhoI site found at 879
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1228
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 257
    Illegal BsaI site found at 516


Design Notes

none


Source

Kluyveromyces sp. IIPE453 and Escherichia coli

References